LOCUS       BG084506                 503 bp    mRNA    linear   EST 18-DEC-2003
DEFINITION  H3101G01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3101G01 5', mRNA sequence.
ACCESSION   BG084506
VERSION     BG084506.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished (2001)
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12567070.
            Other_ESTs: H3101G01-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3101  row: G  column: 01
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3101G01-5"
                     /db_xref="taxon:10090"
                     /clone="H3101G01"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT           97 a          150 c          163 g           93 t
ORIGIN      
        1 cgggcgtgat ccccgggagg cgactacgga caaactgctt cactcggtgg ctccggtggc
       61 tacgtaccat gcaggcgctg ctctgcgcgc tagccgggct cgccctgctc cgtgccggga
      121 cgggcgaatg gggccaaggt cccagggaca cccccggacg gcgagctgcc gagtccccca
      181 gttcccctgg agatctagct gggagcccag gctgtgacag acacgcggct gtgcaaaggc
      241 ggttagacat tatggaggag acggtggaga agacagtgga gcacctggag gcggaagtga
      301 caggtctgct gggcctgctg gaggaactgg cttcaaacct tcccacaggg cccttcagcc
      361 ccaaacctga cttgcttgga gatgatggtt tctgacttcc agggatggtg gagcctgcca
      421 gctgaagtca tccctcagag aaccaagcca ggtcttcctg ccttcctgcc ccacctttgt
      481 gtgaaataaa agctccgatt tgg
//