LOCUS       BG083640                 582 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3090E01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3090E01 5', mRNA sequence.
VERSION     BG083640.1
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3090E01-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3090  row: E  column: 01
            Seq primer: -21M13 Reverse
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          178 a          137 c          134 g          133 t
        1 tttttttttt tttttttaaa agaatccata cccacctttt attacagtgt gtgtagctta
       61 ctgagtgata ccagtgagac aaggagacat ttcaaccact gagaggtcag tgttgaccta
      121 tgaactccaa aagaacaaag gggcttgaca cggatacaac ttacaacctt tctgtgtttc
      181 cagaaactca tggaagcacg ctgggtacta ttacaggcag gaaaattgaa caagcaggcc
      241 agggcaggag gcagggaatg ggagtagggc tgtaggtcct gttccagtgt gcactgggca
      301 atacaatacc aaaacaacaa ccacagagaa aaacaaccca tgaggagctg aactcactct
      361 tgtaataaaa aaattaaaaa tttgttgcct ctgagcacag aacagaccag gaacttcccc
      421 aggcagagac agaattggca agaaatatca cagtggctat caagactcgg tagtgtgcac
      481 tcccctgagc cgacatcttc agataggagg tgcaccaggc ctgtcaaccc agcctggccc
      541 catgcctctg ccccacatgt gtttcttgcc aatccgggtg gc