LOCUS       BG083228                 656 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3085H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3085H10 5', mRNA sequence.
ACCESSION   BG083228
VERSION     BG083228.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12565796.
            Other_ESTs: H3085H10-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3085  row: H  column: 10
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3085H10-5"
                     /db_xref="taxon:10090"
                     /clone="H3085H10"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          179 a          138 c          175 g          164 t
ORIGIN      
        1 atcatatgtg agcgcagtcc cccctttaca gctcggattt atgctgcagg gtttgattca
       61 tcaaaaaaca tctttcttgg ggagaaggct gccaagtgga agacatctga tgggcagatg
      121 gatggcttga ccactaatgg agttcttgtg atgcatccac gtaatgggtt cacagaagac
      181 tccaaacctg gaatatggag agaaatatca gtatgtggga acgtcttcag tctgcgtgaa
      241 accagatcag ctcagcagag aggaaagatg gtggaaattg aaaccaatca gctacaagat
      301 ggctccttaa ttgacctttg tggtgcaacc ttgctgtggc gtactgcaga aggcctttcc
      361 catactccta ctgtgaagca cttagaagct ttaagacagg agatcaatgc agctcggccg
      421 cagtgccctg tagggttcaa cacactagcc ttccccagta tgaagaggaa ggatgttgta
      481 gatgaaaagc aaccatgggt atatctaaac tgcggccatg tccatggtta tcataactgg
      541 ggaaacaaag aagaacgtga cggcaaagat cgtgaatgtc ctatgtgtag gtctgttggt
      601 ccctatgtcc ctctgtggct tggatgtgaa gctggatttt atgtggacgc cggccc
//