LOCUS BG080968 604 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059F01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059F01 5', mRNA sequence. ACCESSION BG080968 VERSION BG080968.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 604) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080968.1. Other_ESTs: H3059F01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: F column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..604 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059F01-5" /db_xref="taxon:10090" /clone="H3059F01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 145 a 160 c 176 g 123 t ORIGIN 1 gctcctcggg tttgtgtctg caggtgccat ccgcgaagat gcagctgaag ccgatggaga 61 ttaaccccga gatgctgaac aaagtgttgg ccaagctggg ggtcgccggc cagtggcgct 121 tcgccgacgt gctagggctg gaggaggaga ctctgggctc agtgccatcc cctgcctgcg 181 ccctgctgct cctgtttccc ctcacggccc agcatgaaaa cttcaggaaa aagcaaattg 241 aggaactgaa gggacaggaa gttagcccta aagtttactt catgaagcag accatcggaa 301 actcctgtgg taccatcggg ttgatccacg cagtggccaa caaccaagac aagctggaat 361 ttgaggatgg atccgtcctg aaacagtttc tgtctgaaac ggagaagctg tcccccgaag 421 atagagccaa gtgtttcgag aagaacgagg ccatccaggc ggcccatgac tccgtggccc 481 aggagggcca gtgtcgggta gatgacaaag tgaatttcca ttttattctg ttcaacaacg 541 tggacggcca tctgtacgag ctcgatgggc gaatgccctt tccagtgaac catggcgcca 601 gctc //