LOCUS BG080472 596 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054B01 5', mRNA sequence. ACCESSION BG080472 VERSION BG080472.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 596) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080472.1. Other_ESTs: H3054B01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: B column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..596 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054B01-5" /db_xref="taxon:10090" /clone="H3054B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 159 a 143 c 161 g 133 t ORIGIN 1 gtcacgagaa acggagcccc ctgcgaaccc cctgtgatga tgacaatcct ccagtgtcct 61 tcgtgaagtt ctctccaaat ggcaaataca tcctggctgc aactttggac aacacactga 121 agctctggga ctacagcaag gggaagtgcc tgaagacata cactggccac aagaatgaga 181 agtactgcat atttgccaac ttctccgtga caggcgggaa gtggattgtg tctggttctg 241 aagataacct ggtgtatatc tggaatctgc agaccaagga gattgtgcag aagttgcagg 301 gtcacacaga tgttgtgatt tccacggctt gtcacccgac agagaacatc attgcctcag 361 cagcgttaga gaacgacaaa acaatcaaac tgtggaagag tgactgctaa gtcctggctc 421 catgggagac ttaaggaagc cacccggatt ggcaagaaac acatgtgggg cagaggcatg 481 gggccaggct gggttgacag gcctggtgca cctcctatct gaagatgtcg gctcagggga 541 gtgcacacta ccgagtcttg atagccactg tgatatttct tgccaattct gtctct //