LOCUS       BG080396                 654 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3053B08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3053B08 5', mRNA sequence.
ACCESSION   BG080396
VERSION     BG080396.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12562964.
            Other_ESTs: H3053B08-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3053  row: B  column: 08
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3053B08-5"
                     /db_xref="taxon:10090"
                     /clone="H3053B08"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          175 a          139 c          165 g          175 t
ORIGIN      
        1 cgcatcctgg gtgaaccggc tgtttctccc aacttgtaga aggggctgtg cttgggacaa
       61 gggaaccttc ctacggaggc atagagagga aggctgaggc tccggggaag atcccaccgc
      121 ggaaacgggt acaagagtgc gtcctttcct gtccttgtca gccccttggc ctttggattg
      181 gcgtcaaaga tcagttgctc tttaaatatt taagccgcga tatgggcttg ctggacagac
      241 tttcaggttt gcttggcctg aagaagaagg aggttcatgt tctgtgcctg gggctggata
      301 acagtgggaa aaccacaatc attaacaagc tgaagccttc caacgctcaa tctcaagata
      361 tagttccaac cataggattt agcatagaga aattcaagtc ctccagtttg tcttttacag
      421 tgtttgacat gtcaggtcaa ggaaggtcca ggaatctctg ggaacactat tataaagatg
      481 gacaagccat tatttttgtc attgatagta gtgataagtt aagaatggtt gtggccaaag
      541 aagagcttga tacccttctg aatcacccag atattaagca ccgtcgaatt ccaatcttgt
      601 tctttgcaaa cagaatggac cttagagact cagtgacctc tgtgaaagtg tctc
//