LOCUS BG080335 707 bp mRNA linear EST 12-MAY-2010 DEFINITION H3052D07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3052D07 5', mRNA sequence. ACCESSION BG080335 VERSION BG080335.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 707) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG080335.1. Other_ESTs: H3052D07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3052 row: D column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..707 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3052D07-5" /db_xref="taxon:10090" /clone="H3052D07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 246 a 155 c 136 g 170 t ORIGIN 1 gctacttggg aggctaaggc aggagaatta caagttgaag gtctttgtgg gctgtaaggt 61 aagttcaagt taggtaactt agcaagaccc ttccttagaa aaggcaggag agctggaggg 121 atggctaagc agtcaagaga cttgagactt cccagcatct acatgaggct gctcacaacg 181 gcctggaacc tcagctccaa gggttccaat gtcctcctct gacctccaca ggtacctgaa 241 cacatgaaca cacacacaca cacacacaca cacacacaca cacacacccc acacacacac 301 atcacatata catacataca tatacataca tacatacata caacacaacg cacacacaat 361 ttaaaaagct ttaagtaaga tgagaaaaga tatggttcag tggtagggag cttgcctagt 421 atgttcaatt tctcagtcct gcccagccct atccctcact gaaaaaaagt aaaactataa 481 cttgaaacta ctaattaaga taaaacagaa atagcaataa tggtctatat gctagttgac 541 tgactagtca atgattaatt aatgttagtc aggtatggtt atttccatct gttatctaag 601 tagtctagag gctgaaacag gaggcttaca aggaatttga agctgtcttg ggcaacataa 661 taagtttcag agcagattag gctataatat gagatcctac tcttaaa //