LOCUS       BG079129                 637 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3036B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3036B01 5', mRNA sequence.
ACCESSION   BG079129
VERSION     BG079129.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG079129.1.
            Other_ESTs: H3036B01-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3036  row: B  column: 01
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3036B01-5"
                     /db_xref="taxon:10090"
                     /clone="H3036B01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          203 a          113 c          104 g          217 t
ORIGIN      
        1 ccatatttta catgggttct ggggatttga atttaggtcc tcatgtttat aaagtaacag
       61 ggcttagcca ctagatcatg ctttatcccc acttgaccac ttgtaatttt tatttttgtt
      121 tgcatcagtg ttttgcctgc atggagattt gtatgagagt gtcagataca ctggaagtga
      181 agttgcaata tgagcagtca tttgggctct gggaactgaa tccaagtcct ctggaagaac
      241 agccagtgct cttaactgct gagccttcta ctagtgattt gaacactttt acattaaata
      301 attataatgc ttactctgct gttctattta atactattta agacagactc aatcccttca
      361 tgttttaata aaacaaatca aacatgacca aaaatcatct atgctatgta attataccaa
      421 gaagagcatt cattcaccta gtaatcaatc tataaataaa atatatacct gtcttcaggt
      481 aaatcattct tttctatggc aatttcagaa gcaacattca atttggaaac ttcattaact
      541 tttcctgtat cttcattaga aattgctgca agctggttct ctaaaaaaaa ggtattagtg
      601 tattagttat caaaagaaat cagagagcta aagacag
//