LOCUS BG079129 637 bp mRNA linear EST 12-MAY-2010 DEFINITION H3036B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3036B01 5', mRNA sequence. ACCESSION BG079129 VERSION BG079129.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 637) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG079129.1. Other_ESTs: H3036B01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3036 row: B column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..637 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3036B01-5" /db_xref="taxon:10090" /clone="H3036B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 203 a 113 c 104 g 217 t ORIGIN 1 ccatatttta catgggttct ggggatttga atttaggtcc tcatgtttat aaagtaacag 61 ggcttagcca ctagatcatg ctttatcccc acttgaccac ttgtaatttt tatttttgtt 121 tgcatcagtg ttttgcctgc atggagattt gtatgagagt gtcagataca ctggaagtga 181 agttgcaata tgagcagtca tttgggctct gggaactgaa tccaagtcct ctggaagaac 241 agccagtgct cttaactgct gagccttcta ctagtgattt gaacactttt acattaaata 301 attataatgc ttactctgct gttctattta atactattta agacagactc aatcccttca 361 tgttttaata aaacaaatca aacatgacca aaaatcatct atgctatgta attataccaa 421 gaagagcatt cattcaccta gtaatcaatc tataaataaa atatatacct gtcttcaggt 481 aaatcattct tttctatggc aatttcagaa gcaacattca atttggaaac ttcattaact 541 tttcctgtat cttcattaga aattgctgca agctggttct ctaaaaaaaa ggtattagtg 601 tattagttat caaaagaaat cagagagcta aagacag //