LOCUS       BG078876                 657 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3033B05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3033B05 5', mRNA sequence.
ACCESSION   BG078876
VERSION     BG078876.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12561444.
            Other_ESTs: H3033B05-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3033  row: B  column: 05
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3033B05-5"
                     /db_xref="taxon:10090"
                     /clone="H3033B05"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          185 a          127 c          161 g          184 t
ORIGIN      
        1 atttaccaca cgtctttttt atcttttaag cagtttgcca ggtgcctctg cagtgcacct
       61 accttgggat ctgaatagtc caccactggg tcctgatgtc tagtgagcag ggattagtaa
      121 aaaggcctta ggtgggaggt gaaaaatgca aggctctcta tttctaggga accttggaga
      181 acctgagtct tacagtggcc agaattctag ggccagggtg gtggatgtaa ttttgtgtct
      241 ttattgaaag ccaaatactt taagaagttt aaaagatttt caaaataaag gtgagggaag
      301 cacagggcct caccatgcaa ggtgacccac ttgagacagt ccacttactg tcataacagg
      361 aagctcttga gacactgagg gaagacagcc cagggcagaa caaagaagag ctggcgcttc
      421 ttttccctct tgttagaaga cttttttaaa aagtagcctg gcttgcatgg accttgtttt
      481 ctgaacagct tcaggatgca gttgtggtag atgtttagtg actagccagg attgatggtt
      541 tcccaccatt ttaaaccaag taaccctcaa ctcaaattca ctttgaagtt ggataggaat
      601 ttaggatctt tagtaagaag tgtagaacat tccccagcac ttagaaggtg gaggtac
//