LOCUS       BG078819                 587 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3032D01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3032D01 5', mRNA sequence.
ACCESSION   BG078819
VERSION     BG078819.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12561387.
            Other_ESTs: H3032D01-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3032  row: D  column: 01
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3032D01-5"
                     /db_xref="taxon:10090"
                     /clone="H3032D01"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          143 a          131 c          151 g          162 t
ORIGIN      
        1 gtgggaccaa ggctacaggc aagcttgcac accagccatc cctctcaagt ggcaggggcc
       61 ggtgcctaga gcaatagtgg atttcttgtc ctgggctgtg ttagttgttt agatttaatg
      121 ggtcagtaaa ggaacaggga ctcagaggct ggcccatggc cacctttgtt ggcatgggtt
      181 tctattctga caaagaatgc tttgtaagag ctgcttttta gggattatct tttaaacacc
      241 ctgaatgggg aacagggttc tcctctacca cctcaaccag agtcatagga aagaggtcct
      301 attttcctgc tgcttcacag ctcagaacat gtattgaatg gaatgtattt ttaagagaat
      361 aaagtctatt tttttgtatt ttaaagattg agagggctag aaaggtagat agcccttaaa
      421 taaacttacg catgataggt cctttgctgg ggcctcagct gttggtctac acctgcccag
      481 gcaggtggct ctggcttcac ctctgcccct tggagtctag gtgcagcagc ttctacattc
      541 ctgctccaag cacgggacca gagaggccag aacctgcctc tggaaag
//