LOCUS       BG078790                 550 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3032A02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3032A02 5', mRNA sequence.
ACCESSION   BG078790
VERSION     BG078790.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12561358.
            Other_ESTs: H3032A02-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3032  row: A  column: 02
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3032A02-5"
                     /db_xref="taxon:10090"
                     /clone="H3032A02"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          144 a          114 c          134 g          158 t
ORIGIN      
        1 ctggtggtca gttcaagcac tgcccgtggt tgccagacaa gcctttcagc tcttttatag
       61 agccagacct cactatagtc caggtttaga ccataatcct ctgctgcccg gctaattcct
      121 ttgaatttag tcctcagcat cccagatgct ggggttacac atgaacagtg tcacacctgg
      181 cttatcttgg ctttttaaat ataacatcct aaaaccatag agttcttaaa tgtatgtggt
      241 aatggcttca gggcccaggc tgtgacacat gaatctcagg ttaactcagg aagacctatg
      301 aaactaacgg actcttggag gctgtttccc ttggggttct ctctgtttaa aaggtgatac
      361 aagagatggg aagatggttc agttagtgta tgtggtacaa gcataagaac tgcgtagaaa
      421 gcctggagta gctggttgta atggtgtatg ctacatcttt aatcttggca tttgtgaggc
      481 aggactactt tgtgagctgg tctacataga gaactccagg actgcataga gaggcactgt
      541 ctcaaaggaa
//