LOCUS BG078133 599 bp mRNA linear EST 12-MAY-2010 DEFINITION H3024A06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3024A06 5', mRNA sequence. ACCESSION BG078133 VERSION BG078133.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 599) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078133.1. Other_ESTs: H3024A06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3024 row: A column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..599 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3024A06-5" /db_xref="taxon:10090" /clone="H3024A06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 139 a 133 c 175 g 152 t ORIGIN 1 ctagcagatt tacctgcaaa ggaaagtctg tttttcactt catggggact agcacatttt 61 ccgagtacac agttgtggct gacatctctg ttgctaaaat cgatccttcg gcccctttgg 121 ataaagtctg ccttctcggc tgtggtattt caactggcta cggggctgct gtgaacactg 181 ccaaggtgga gcctggttct acttgtgccg tctttggcct gggaggagtt ggactggcag 241 tgatcatggg ctgtaaggtg gctggtgcat cccggatcat tggtatcgac atcaataaag 301 acaaattcgc aaaggccaaa gaatttggag cctctgaatg tattagcccc caagacttca 361 gtaaatccat ccaggaagtc ctcgttgaga tgacagatgg gggcgtggat tactcctttg 421 agtgcattgg caacgtgaag gtcatgagat cagcccttga ggcagcccac aaaggctggg 481 gtgtcagtgt ggtagtggga gtagctgctt ccggggaaga aatctccact cgtccattcc 541 agctggtgac aggacgcaca tggaaaggca ccgcctttgg aggatggaag agtgtggac //