LOCUS BG078131 593 bp mRNA linear EST 12-MAY-2010 DEFINITION H3024A03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3024A03 5', mRNA sequence. ACCESSION BG078131 VERSION BG078131.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 593) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078131.1. Other_ESTs: H3024A03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3024 row: A column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..593 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3024A03-5" /db_xref="taxon:10090" /clone="H3024A03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 109 a 170 c 196 g 118 t ORIGIN 1 ccacgctgcc ctggaccgac tatgtgccca tcggtccgtg ggattcggct gccaggagag 61 ccgtcactcg taccccccgg ccctgggcag ccccggagct ctaaccggag ccgtggtggg 121 aacagcgggt cctttggaga gacgtggggc gcaacccgga cgacactcgg ttacaggcta 181 cggggactgc gccgcggggg cccgttacca ggacgagcta acagcattgc tgcgtttgac 241 cgtggctacc ggtgggcgag aagcgggtgc tcgcggggaa ccctcgggga ttgagccgtc 301 gggtctggag gagtctcctg gtcccttcgt tccagaggcc tcccgatcac ggatacggga 361 gccagaggcc agagaagatt actttggcac ctgtatcaag tgcaacaaag gtatctatgg 421 gcagagcaat gcctgccagg ccctggacag cctctaccac acccagtgct ttgtctgctg 481 ctcctgtgga cgaactttgc ggtgcaaggc tttctacagc gtcaatggct ctgtctactg 541 tgaggaagac tatctgtttt cagggtttca ggaggcagct gagaagtgct gtg //