LOCUS BG077777 646 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019F08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019F08 5', mRNA sequence. ACCESSION BG077777 VERSION BG077777.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 646) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077777.1. Other_ESTs: H3019F08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3019 row: F column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..646 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019F08-5" /db_xref="taxon:10090" /clone="H3019F08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 170 a 124 c 121 g 231 t ORIGIN 1 cttttcaagg tatcctgact tttacaaagc atatatattc aggacttaga aaatagcagt 61 acacatttaa taatagagtt atatatcaag atgttttact ttgggattta gatgtgtttt 121 gtgacagatg agaatataca agtcatgcat tctcttaatt gacaatttgt tagaatttca 181 ggattacctg gtatccccat agaatgttat gcgatctttg ggactaaata ctacaagcag 241 ctctttgtag cagtgtttgc actgaatgtt ttagagtcat ggttgctgtc tttttagttc 301 cttctttgtc ttttcctact gtggtgatta agggtcctgt tgtggttttg gaattagccc 361 ttatgatcca ggcatagtag cacacagctg caattgaagc actggaggaa actgatggtt 421 gtgactttaa acccagcctg gactgcagac catgctccat gctagtcttg ctaagatcct 481 gtgtccttcc ctttccccag ttaatctact atcaacttta aagtcttcat gtcatcaagc 541 agagcattta cttgagtata ttatcgtgca gttcctcatc tagtcccata atgatctttt 601 gtgcctcagt gaatctactt gtaaactcca agcttatctc ttagaa //