LOCUS       BG076659                 569 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3004C03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3004C03 5', mRNA sequence.
ACCESSION   BG076659
VERSION     BG076659.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12559228.
            Other_ESTs: H3004C03-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3004  row: C  column: 03
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3004C03-5"
                     /db_xref="taxon:10090"
                     /clone="H3004C03"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          124 a          110 c          163 g          172 t
ORIGIN      
        1 ggggggcttg tggagtagtt ggtaatagtg aggggttcct cacgtcactg gaagtgggtt
       61 ctgagttgct ggagaaagta gaaagatgaa tgtttttaaa tgaaagtgtg tgtggggtgt
      121 gtgtgtgtgt gtgtgtgtgt gtgtgtgtga gtgactgtgc tcctggaaca gcacctggga
      181 agtctctagt cttctctgag gattttgacc agtaggctac cacagaaaga gttcggtatg
      241 gtgaagcctg gtatttcaag tggtgcttgg gaaggagcct ggctcggtgt ccactgttca
      301 acaccttgcc tccagccaca gtcctcagtt tcatttttac agccttctct cttccgtgct
      361 tctgggaaag ttaacattgg gccctgtttt ggaagtaacc aaggtttaac ccactaaagt
      421 ttcacgtgct ccttgctact gagattggtt caagttgtct taaacttaag acatgtccca
      481 agccaaactg cagagtcagc aagccatgat tctgggtatt tgcatattgt ggaggctgag
      541 caaggggctc tgtactctcc caaactact
//