LOCUS BG075996 652 bp mRNA linear EST 26-JAN-2001 DEFINITION H3154C10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3154C10 3', mRNA sequence. ACCESSION BG075996 VERSION BG075996.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 652) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3154C10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3154 row: C column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..652 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3154C10-3" /db_xref="taxon:10090" /clone="H3154C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 235 a 135 c 133 g 149 t ORIGIN 1 aagatggtga catttccttg actctgtctc tcaggcctca cctaaatggg tggacggata 61 gatagatttc agacagatgg acagatggat ggacagataa acaaataaaa attttaaatc 121 cccttaaaga cagagaaact gcctggtatt ggaaacctag ccaactacct tgggctaggg 181 aagtcatgct tcttgacaga gaacctacaa ccactaattt actaaataat atatatagtc 241 cataactatg ctctaaattt ataccaatag ataagcatag tgcttatccc tcatcaagga 301 aatttctctt tgtagcataa ggaaaacatt gcagaaaacc acaacagttc aaaacacaga 361 gttgtggaac tcagttccaa ctgagccatc tacaacacaa taactgcacc tacagctcag 421 ggaccattgt agacgaaggc ttagagactg taagaggtag aggatctggg agtttgctgt 481 gagattgtct actggcaatg tcagaagcta cacccataaa gtctcatcaa catgactacc 541 aaacatgagc tgagcaagga gaacaaaaga catgccagaa tgggagaaag ctgaccaagc 601 ctcaacccta gacaaagatc tactggtaac taaggaatgc taagagagca tg //