LOCUS       BG075996                 652 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3154C10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3154C10 3', mRNA sequence.
ACCESSION   BG075996
VERSION     BG075996.1
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished (2001)
COMMENT     Other_ESTs: H3154C10-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3154  row: C  column: 10
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3154C10-3"
                     /db_xref="taxon:10090"
                     /clone="H3154C10"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          235 a          135 c          133 g          149 t
ORIGIN      
        1 aagatggtga catttccttg actctgtctc tcaggcctca cctaaatggg tggacggata
       61 gatagatttc agacagatgg acagatggat ggacagataa acaaataaaa attttaaatc
      121 cccttaaaga cagagaaact gcctggtatt ggaaacctag ccaactacct tgggctaggg
      181 aagtcatgct tcttgacaga gaacctacaa ccactaattt actaaataat atatatagtc
      241 cataactatg ctctaaattt ataccaatag ataagcatag tgcttatccc tcatcaagga
      301 aatttctctt tgtagcataa ggaaaacatt gcagaaaacc acaacagttc aaaacacaga
      361 gttgtggaac tcagttccaa ctgagccatc tacaacacaa taactgcacc tacagctcag
      421 ggaccattgt agacgaaggc ttagagactg taagaggtag aggatctggg agtttgctgt
      481 gagattgtct actggcaatg tcagaagcta cacccataaa gtctcatcaa catgactacc
      541 aaacatgagc tgagcaagga gaacaaaaga catgccagaa tgggagaaag ctgaccaagc
      601 ctcaacccta gacaaagatc tactggtaac taaggaatgc taagagagca tg
//