LOCUS       BG074503                 586 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3135H09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3135H09 3', mRNA sequence.
ACCESSION   BG074503
VERSION     BG074503.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG074503.1.
            Other_ESTs: H3135H09-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3135  row: H  column: 09
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3135H09-3"
                     /db_xref="taxon:10090"
                     /clone="H3135H09"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          204 a          100 c          124 g          158 t
ORIGIN      
        1 gagatgaaac agtttttatt gctaaattat tacaaagaca ggttgttgaa gatggttaca
       61 attaatttat gctgcatgat ttcaagacct ttacttcata attaggggtt ggagattggt
      121 ccaggacccc acgaaccaga aacatcaagg tagaaaccag aacttcaaag acagccccac
      181 agccagctga gcttcaagtg agttttaaat taattggtcc ctttgaggaa ggtctggctc
      241 agaagaaaca ggaactagta gaactgtccc ctgctcacag tgagtagcca gagaaagcat
      301 ctaagaggac ctgaagcttt tgaatgaaag ctggttcaga aaggttctgt tgtaaaggct
      361 ttgctgtctg tttgagacca aattgcatag cagtgccatg aggtccaggc atgagaccat
      421 tgggttataa atatataatc ttcagaatga caggcactta aaactaagta aaatacaaaa
      481 atagatcata ttaataggaa ctatttagta tttaacaaat tgtaaatcca gggttttacc
      541 atttataaaa ggatcctagg aactgaagcg ctgctatcca aataaa
//