LOCUS       BG073018                 669 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3117F09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3117F09 3', mRNA sequence.
ACCESSION   BG073018
VERSION     BG073018.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12555587.
            Other_ESTs: H3117F09-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3117  row: F  column: 09
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3117F09-3"
                     /db_xref="taxon:10090"
                     /clone="H3117F09"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          191 a          157 c          117 g          204 t
ORIGIN      
        1 atcttggcag ttagaagcct actctgtgta tgcctaggcc ttagcttcta ataccaccat
       61 ctaaaaatac atcttgccac aacatgcttc gtctcctgta ggtgggccat ttaaaaattg
      121 acaccgaagc aacgtaccat aggaccactg tcctttgatg cactcagcct gtatattaaa
      181 gactcagaaa tgaagttatt acaatttaca ggaaaaaaaa tgaattcaca tgagtggctc
      241 aaatggtgtt tctagtcccc tttttttgtg gggggtgctg aggttatgta atcagacata
      301 attgtctgca catgtttgtt acactatctc agaaatgatt tccagcccac cagatgcaga
      361 agggtcaata ctggaagacg cccccatcaa gcactgctta ctatagtact gcagttaggg
      421 attaaaaata tgccaaataa gaaactgtca ggcccacttt gtactgactc ataaagactt
      481 ccttcaggtt ttttttttcc agacacctat atttttgttc ccacttctta tacattcttc
      541 atattcttct ctttcattta atgcacaaaa tcatttctgg agaagcctag catctcagaa
      601 acgctcagcc atacccagat gcaagtgcag ttaattccct agccagccgg cttcttcctt
      661 tactgggac
//