LOCUS       BG072867                 723 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3115H07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3115H07 3', mRNA sequence.
ACCESSION   BG072867
VERSION     BG072867.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG072867.1.
            Other_ESTs: H3115H07-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3115  row: H  column: 07
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3115H07-3"
                     /db_xref="taxon:10090"
                     /clone="H3115H07"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          152 a          226 c          176 g          169 t
ORIGIN      
        1 aacttttgaa ttattttatt cccatagttt gacatgatat acaggcactt tggccaatga
       61 ggtggcacag gccactggct cctgcctgag tcagttaccc accttcaagc cctctgagga
      121 ttagtgctag taatgtcaca cacaaaatgg ggggctaggc tggggaatgg aaagacaaga
      181 gggggaatcc acagctgtcc tggggcttca gatggcagca gcctgggttc cctcaatcct
      241 ggtggaacct ggaactgctg tttctgaaaa tcccacactg ggcagtcgga ccctgaatcc
      301 accttcttcc cggtccctac tcccactccg ggccttgggg cttaaggaca ccctaggaaa
      361 acggaatttg ggtgacttct ccccaactgg ggacttggag gttgcatcac cctcctgtcc
      421 cttagagact ttagaagggg aagccaagcc tacccgaggc aggcggaccc tcaccctacc
      481 ccggcgactg gaagcccctt ccccactgcc ttcttcctcc tcctcaggac ttggagagcc
      541 ttctccggag accgactcac tctggctgaa gcccactcgg ggtagcctga gctttggact
      601 cttagccttt tctccaactt ctatcccttc cttggccttt gccagaccaa acaggggcag
      661 ccgaaccttg agtttgtgcc caccatcccc atcaccctca actacctggt actcagcgtg
      721 gct
//