LOCUS BG072727 695 bp mRNA linear EST 12-MAY-2010
DEFINITION H3114C07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3114C07 3', mRNA sequence.
ACCESSION BG072727
VERSION BG072727.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 695)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072727.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3114 row: C column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..695
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3114C07-3"
/db_xref="taxon:10090"
/clone="H3114C07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 274 a 114 c 90 g 217 t
ORIGIN
1 ctaaatgaat atctttttat tgaattttcc aattaaaaat gaaatttaat tttacatcta
61 taattagaaa tgacacacac acaatgggac tgtacaaatt taggtcaaaa taaaaatata
121 tatatcttgt ggcttcataa aacatcattt actaaatctt taaagaaagc agaagttaac
181 agcaatgtgt gtaaaagtaa tgatttaatg gctacaagca agacaaagca atacaattgt
241 gcttcttctg cagactgcag acaatgaaat gcttagctac agtttcccat acaaacatga
301 aacaatattc atacggaatt aacatcctca caaataactg atggctgata aacacacgct
361 aagttcgacc aaagcaaaac tgagacttgg aggggtcatt catattttaa attcaacatg
421 tttgctctat taaaaatccc tgtagaaatc ataacaaatt gtttctgttt ccagacgtta
481 cagtgaaggc agattccttt aactatgaaa acaactctta aatttgaaat tacacttgtg
541 ccacatacac taaaataaaa ttgtagataa aatgctttac ttcagcaatt agctggttga
601 ctttaaaatc tattttagaa ttccactgat aaacagaatt taaaaacatt aattctattt
661 taaccaatgg aaaattaaac actatatttg ccttg
//