LOCUS BG072671 577 bp mRNA linear EST 12-MAY-2010
DEFINITION H3113E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3113E12 3', mRNA sequence.
ACCESSION BG072671
VERSION BG072671.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 577)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072671.1.
Other_ESTs: H3113E12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3113 row: E column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..577
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3113E12-3"
/db_xref="taxon:10090"
/clone="H3113E12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 154 a 108 c 168 g 147 t
ORIGIN
1 gtaaagactt gatttgattt gtgtgcactt gtttgtgtgt gtttctgtgt gggggagggg
61 gtggagtggg ggaagccagg aagggacact ggaccccttg tggctggagt tatgggtgtt
121 tggtgtttgt gagctgctcc ctagaggtgt aggtgctaga gtgagaactc tggtcctctg
181 atggagcagt cagtgctctc aaccacagag ctatctctcc agccccaaca cacttgactt
241 ttacagcagg cctggacaca caccacagtt gtattccctg tgtatgtgtg gtgtgtaagg
301 aactacacag taggcaccaa gcctacacct atgactatac gctgagaagc aacagggaaa
361 gagagcatgc tttagttata gaaagcacgt tagggctgag ctatgtggat gtacaatgac
421 cagggaaggc acaaaggaac cttgcagtga tggaaatgct ctgtatcttg attgggtgac
481 aataatgaca cagtaaacac atagggcaga gctcactgga tggaaaaacc caaaacagat
541 gcgatttatt tctatagaag ctgtgtttca atagagg
//