LOCUS       BG072397                 822 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3110A11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3110A11 3', mRNA sequence.
ACCESSION   BG072397
VERSION     BG072397.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12554966.
            Other_ESTs: H3110A11-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3110  row: A  column: 11
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3110A11-3"
                     /db_xref="taxon:10090"
                     /clone="H3110A11"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          247 a          206 c          167 g          202 t
ORIGIN      
        1 gctttttgag acagggtttc tctgtgtagt cctggctgtc ctggaattca ctttgtagac
       61 caggctggcc tcgaactcag aaatccacct gcctctgcct cccaagtact gggattaaag
      121 gcgagctcca ccacgcccag ccatatacaa atttcttgga taagaagggg tttcaagtaa
      181 aaaaagttta agaagtcatg aaccaggagg ctggaggaat accctggtcg ttaggagcac
      241 tggctgctct tccagaggcc ctagatttga ttcctagcac ccacatggaa gcttataact
      301 ttaactacaa ttccaggaat ctgacaccct cttctggcct ttgcaggcac cagggacaca
      361 tgatatacag acatacaaac aagtaaaaca ctctccacac acgaacttaa aaacaaacaa
      421 acaaaatctt tttaaaaagt catgaatcag agtgttccct tttgggagta accccaaaga
      481 tccctttctt gaaatctgac aacttaaggc tggaaagatg gcagagacag gcaggcctct
      541 ggggcacact gaccgcctgc ttaggtatat ctatgaatct atgagttcca ggttcagtga
      601 cagactctgt ctcaagaaaa ttaggttgag agtaatcaag gaagacacct aatgtcatgt
      661 acccacatag acaaataaaa cacggtgtgc ctaaactccc cactgaaaaa cttgccaccc
      721 tgaataggag tctcaacttc ctcaggccat cagacttcct cagactgcca gttttttgta
      781 gagaccctct gactagcact tccacactca gttatttgtg gc
//