LOCUS BG071841 893 bp mRNA linear EST 12-MAY-2010
DEFINITION H3103G07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3103G07 3', mRNA sequence.
ACCESSION BG071841
VERSION BG071841.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 893)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071841.1.
Other_ESTs: H3103G07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3103 row: G column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..893
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3103G07-3"
/db_xref="taxon:10090"
/clone="H3103G07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 232 a 228 c 174 g 259 t
ORIGIN
1 tttttttgaa gctccctccc cacagccact tgatggggtg gggccaaggc tgagagtgga
61 agccctggag tcctgggccc atgtcttcat ccataaactt tctcttcttc agggtgctga
121 gcacaccagc tgcaacagta ggctctatta atcattcctc catccccatt ctctccagcc
181 atctaccacc aaagcaaggt cccattggca gctataaaat gcacttccta cgcttactat
241 aaagaatgac taaaataact gcttttccca tctttgaaac attacttatt tattcattta
301 atatgcatgc atgtttaatc cgcatgtctg tccatgcaca ggagagattc cctgagactg
361 acctccctgg ggatgctagg aattgaactt aggacccgtg gaagagcagc tggctctcct
421 gaccactgct cagctagctc cccagatctt attttttcct ccgtgtttta aaatcgcttt
481 tggtcaagat agttttcata tttgtgaaat gtaccatgct agctactgct tacctgtttc
541 catgtcacta agccatcaga gctgagttgt caagggccag ttgtttcata ggctgtagac
601 acacagagga atctcttcta atatcaatag gactctcaaa gcatcccatc taaggcacta
661 aggcattcct gcttcttttt aaacagggtc aacacatgtc cctcatccat aaggaaccca
721 gcgacccacg gccagacagg ctgcatcacg gaagagtgca agtgattggg gtaaaagagg
781 aggctagagg tttttgatga ttcttcctgt tctggatttt atttctttta atcgagtttt
841 ttatacaaca cctacatgca cacacacaca cgtgcacata cacatacaat gtg
//