LOCUS BG071439 452 bp mRNA linear EST 26-JAN-2001 DEFINITION H3099A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3099A04 3', mRNA sequence. ACCESSION BG071439 VERSION BG071439.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 452) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3099A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3099 row: A column: 04 Seq primer: -21M13 Forward POLYA=No. FEATURES Location/Qualifiers source 1..452 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3099A04-3" /db_xref="taxon:10090" /clone="H3099A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 110 a 102 c 112 g 128 t ORIGIN 1 gtttgggggt ttttttactt ccaaattctt tgtttattaa acttaggaga cattaaggta 61 ggactttcat ttttaataaa ccccctcaag tgtgcaccat gacttaaaat ataatctaaa 121 tataaagcac tctggttctc catgccttcg gtggggttta acaccggact gaaaggtact 181 ggttcacaaa gggaactctt aaatacacga ctgactcttc tcctgggtcc tgggctccat 241 ggctggtttc agttccaggt agggtttttg gctctcctcc acctaccttc atgggtctct 301 gggagcaata gcacttggtg gttataggac caggtgcaat aatacaaagg aatgggtggc 361 ggccatcccc tcctcccttc ggatgactgg gaagcggcga accctgaatg agatggctct 421 ggacctcgca ggtggggcag aaccagggaa at //