LOCUS       BG070613                 548 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3089C02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3089C02 3', mRNA sequence.
VERSION     BG070613.1
DBLINK      BioSample: LIBEST_008622
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished (2001)
COMMENT     Other_ESTs: H3089C02-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3089  row: C  column: 02
            Seq primer: -21M13 Forward
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Mus musculus"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          142 a          112 c          175 g          119 t
        1 ctctgaaagt aataaatatt taccatggtc tatatccacg cagcagcgat tgagggaaaa
       61 cgaggaaaga acctttctgt gactctcaat aacgaattct acaaacaggc tgttgcagcc
      121 tttgggaaat ggtggctagc tttggaatac ttgtcatttt aggcagattg gcctgtcata
      181 ttgtcaaaag cacaggccca ggaaggcaag ggatggaagg tgggagaaag ggagtgaatg
      241 aaaacggaag gaagggaagg ggagaaagaa gggtgggaga gtggggtggt ccaggaagtg
      301 atgcagggcc ctccttcgcc cgggttggtc tccttggttt tcttcctctt tctgccttgc
      361 ccagcgttct agccaccagc tggaatgtca ggtggttgat ggtaaggaca agcaggtagg
      421 ggccggcctc agggtagacc caggcaggag gctggtagca ggaagaacag atgacacctt
      481 tgtgaagcag ctgaggacag tccttgctga ggctctgtta cagccagctt ccagctcgac
      541 ggcccgag