LOCUS BG070291 685 bp mRNA linear EST 12-MAY-2010 DEFINITION H3085G05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3085G05 3', mRNA sequence. ACCESSION BG070291 VERSION BG070291.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 685) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070291.1. Other_ESTs: H3085G05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3085 row: G column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..685 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3085G05-3" /db_xref="taxon:10090" /clone="H3085G05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 213 a 157 c 150 g 165 t ORIGIN 1 aaaatattta ttacctagac tcccagttgc aaggcacatt aacaatgtta ctaagacaat 61 gggaacccag agacaaaggc ccctaaggtg tggcgagtcc tgcatcatta acactgcagc 121 tgctgcagca gaggcaagac tgcgtgggga acggctgctg gtcaggcaca gaagggctct 181 gggctgatgc caaggattct ttagacttaa agctaccttg ttttccacat gccttcccac 241 gccctccacc ttacaaactg cttaaagctg tagagagtac atttatttct tgtcaaatgc 301 tgcaggttcg cctgaaagga gtcttgaaag tgtggtccat ccacacggct cagccaaaaa 361 gccaactaaa cagagctgct ccggagaacg atcacagaaa tcccaacttt cttcaccttg 421 gttacggatc cacagtttgc acagaaataa ataatatgca tgaagtcgaa ttaggattaa 481 aacttgaaac aagaataaaa aaggggggag ttgggtgggt ggtataacaa tctggtactc 541 ttatggcaaa acaatgaaaa tgtctttaaa tagacacttt attatatcca caggcgataa 601 aatcccagag ccactgttag gcacgtaaac acagaccccg ctaagtattt gattgtatca 661 agcttgtgcg cctccagcat cgtct //