LOCUS BG069777 394 bp mRNA linear EST 26-JAN-2001 DEFINITION H3080A05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3080A05 3', mRNA sequence. ACCESSION BG069777 VERSION BG069777.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 394) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3080 row: A column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..394 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3080A05-3" /db_xref="taxon:10090" /clone="H3080A05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 120 a 86 c 110 g 78 t ORIGIN 1 cagtacacag attgtaattt attatagtca gtgttatttc aggacaagcg gaaataaaaa 61 ctgcacaagt tgcaggcgag tcgcaaggat gcctctacag tagataccgg aggaccatag 121 atcaagggaa gaaagtgatg agactcgtca tactcacaca agggaatctc aggtcgaact 181 tggggatata ttcgacaaag tctgcgtcat acgcatagct gaatgaaagg ccatgtagcg 241 gggaggacat tttcaccatt acaaatgctc taactgtgaa aggtatgcca gtgctgtcga 301 cctgggtccc cagtgcgagt ctgaggggcg agctgggcca gcctagcggc gacgcgccag 361 ggaaaaggta gcggtcccca aaacacacac agga //