LOCUS       BG069370                 681 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3076F02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3076F02 3', mRNA sequence.
ACCESSION   BG069370
VERSION     BG069370.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG069370.1.
            Other_ESTs: H3076F02-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3076  row: F  column: 02
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3076F02-3"
                     /db_xref="taxon:10090"
                     /clone="H3076F02"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          180 a          170 c          172 g          159 t
ORIGIN      
        1 ctctaaatat gcttttatta gttgacaaat gacacagtct ttacctcatt acacataacc
       61 agccccttgc tgggagtgac aaggtaagac ttgttcaaca aagcatttta ggagaagttg
      121 aagtctcagc ggtgtgtggt agcatattct caccacccag gcattctgga gacaaaggca
      181 ggagcattgc ctggtataca aagtccaggc cagccaaggc tgcatatcaa gaccctaact
      241 cttaggattg gagcgtttta aatccagtgt tgggcaataa ccccaacact ggaggcaggg
      301 gagggctgca agtccaagac cagcctgggc tacgcatgag atcctgtctc aaaacaacgt
      361 gtctcagcat ccttgctgtg tttcagaaag tcattcccag acccacacat ggagtggcat
      421 ctgcttccta tttcgaaaga gtgaggcata ctccacagcc agcaaggatc tcgtgacaca
      481 tggcgagcca ggctggatcg ttcctcgaga atgaggatgg aggagccctg attgtgagga
      541 actgagggag ggggtggacg aacatctcag aacagctgta acggatagag cgggattccc
      601 ttcctccgct agcgtgtgcc gctaggatat gtaaacttta ctaccaaccc cgcccgattt
      661 cacagtctcc gttctcgctt t
//