LOCUS BG069321 816 bp mRNA linear EST 12-MAY-2010
DEFINITION H3075B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3075B03 3', mRNA sequence.
ACCESSION BG069321
VERSION BG069321.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 816)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069321.1.
Other_ESTs: H3075B03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3075 row: B column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..816
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3075B03-3"
/db_xref="taxon:10090"
/clone="H3075B03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 223 a 173 c 196 g 224 t
ORIGIN
1 gtaaaaacat gaggtagttt taatggcaga gctccgggtc gaaatgtatc tcatgcagga
61 gacagtggat tcgaccacaa ggcttggaag ctaggggttt ttatagaaaa ggggctgggg
121 ttgggagaat tggcgaggtt tcacatgatt ggttcattta aacatcagca gactgtacat
181 gcagataaca tttaacttag gtcagaaggg cgggagatag ggaggcgccg ggccagtccg
241 gacatgtcat tctcctttct gcaccacctg caagcctttt aacttagcat acagattatt
301 acaacaacat gtaggtttaa acacatcatt taagacagtg atgggcatgg gagccccata
361 aaggatctta aaaagagtaa ggctgaatca aaagggagta tttctagttc taaatagggc
421 aaggggaagg agcaccaagt ctgcaccagt ctcttttaga attctattca tctttttttt
481 ttacctgtcc ttagctttgg agcctgtatg cacaatgtaa cttctaatca gtccccaata
541 tcttggccag tcgctgattt acctgggcaa caaaggcagg tccattattg ccctgattac
601 cttgggtatc ccaaacctcg ggaagatttc ttctaggatc ttcttggcta tcacattgac
661 cacctcagtc cttcaactgc gaggctactg ccatccatgt accagctcaa actctcaggc
721 caaggctgat ctgatccttc agatcatttg gagtaccagt ttcttctgcc agaatgtcag
781 tgcaatgatg agtaggtgaa gtctcggaca gtaggg
//