LOCUS BG069097 629 bp mRNA linear EST 12-MAY-2010 DEFINITION H3072F12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3072F12 3', mRNA sequence. ACCESSION BG069097 VERSION BG069097.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 629) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069097.1. Other_ESTs: H3072F12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3072 row: F column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..629 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3072F12-3" /db_xref="taxon:10090" /clone="H3072F12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 199 a 109 c 120 g 201 t ORIGIN 1 aatctgttta tttgaacagg gagactagga acaaaggtta aagatattct aaattgattt 61 gaaagaccta ccaaaattac atatttatcc tgatacatta aaatatttcc atggatatat 121 agttgagcta gaaatgcatc aagtgctttg gattataatt actttagaat tagatattta 181 gtgattttta tacattctct gtgatatatt aattgtagta ggtgatgata acatacagaa 241 agtatatgat atgtaaaatt tctgagagag taaatacttg actgtggctg atagcatctc 301 ttgcgacagt tctaacacac aggattggtg gagaacaggg tgtatgagag cccagttcct 361 ccctgttaca actgaaagac agccttgtag tcacaggcag tacctgactc ctctattctg 421 cttgtcacat aatatcagcc agatgaaata atgaggtatg gaaattctca ccactccctg 481 aaaacctata aaagttagaa attccttttg agagggttct ctgaagcttt tgggggtgca 541 gagcatcatg ctgtatagaa caagtatcct tcatggtcag tcacttccag cactccctac 601 tgtcttgttc ttgcatctta caaagctct //