LOCUS BG069010 556 bp mRNA linear EST 12-MAY-2010 DEFINITION H3071G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3071G04 3', mRNA sequence. ACCESSION BG069010 VERSION BG069010.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 556) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069010.1. Other_ESTs: H3071G04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3071 row: G column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..556 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3071G04-3" /db_xref="taxon:10090" /clone="H3071G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 150 a 97 c 95 g 214 t ORIGIN 1 cctgactgcc tttttatagg cttggtcaca gcctatatca tagtctgctc tatagcaagt 61 gtctggactc acttctgtgc taagaaagca gttacttatc ttcttgttgc tgttgctttc 121 aatagccact atagttggct tcatatatat atatgccaac tatatatgta tatatacata 181 tgtatacata tgtacatatg tatatatact atgtgtatat atatgtatat atattcatat 241 gtatgtgtgt atgtatgtat gtatgtatgt atgtatgtat gtataataac tgcctcttct 301 acctataagc tatgtgatga tgatacaact gctggactat atccttatct ccataatcag 361 agccagacac tctggttttt aataaaatac tttagcaaag tttatagtat tatctccaac 421 tatgattcta tgcttttaga aagcaataat ttcctatgtt tgcaccaggt tgggttgtcc 481 aattgtccca ctacagctgt tttggctcag atgatttccc aatctgtcgg ttggccatct 541 gtctgggaga tgtgta //