LOCUS BG068260 699 bp mRNA linear EST 12-MAY-2010
DEFINITION H3063E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3063E03 3', mRNA sequence.
ACCESSION BG068260
VERSION BG068260.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 699)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068260.1.
Other_ESTs: H3063E03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3063 row: E column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..699
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3063E03-3"
/db_xref="taxon:10090"
/clone="H3063E03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 198 a 190 c 153 g 158 t
ORIGIN
1 aaaatttaga aaatgccatt ttaatcccct cactgaaaag gcttctctcg accagattgt
61 aacaaaatcc acagcttatt ccaaaagaaa aatttaatac aagagatttt agaaaattaa
121 acatatttgt ttaaaaattc tatcgtgtaa accttaacat aagagggagt cgggatagca
181 catgctcaga tctctgggat gtttgcaccc gcagctccag acatagggct ggagtagcct
241 cagagacctt tccaggctgg atggagatag cagaccaggg ccttctctcc cctggaggtc
301 ccagctctca ggtcctcttc ccaggtgctc agtgaccctg tgcgaaccac tgtcacgtgt
361 cccatgtccc aattcttgct gggtcctccc aatctgtcct actgatgaaa aagaaaacaa
421 aaacaaaacc aaacctccct gtggtcaagt ggttgcctcc tggtaagggc agagctgagc
481 aggctgcagg tggcactgga acaggtggtg agcgatgtct tcagggcagg agaacatggc
541 cccgtgagaa gtgaacccca ggcctccaca cagcagccac tccagccctg acgctgccct
601 ctctcccctc cccacccact gcctagactt gactttagac gtagaatgga gaccacatat
661 cacagcaaca gagcctgaac tttttggcac agaaatatt
//