LOCUS       BG067986                 619 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3060E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3060E02 3', mRNA sequence.
ACCESSION   BG067986
VERSION     BG067986.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12550555.
            Other_ESTs: H3060E02-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3060  row: E  column: 02
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3060E02-3"
                     /db_xref="taxon:10090"
                     /clone="H3060E02"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          191 a          119 c          106 g          203 t
ORIGIN      
        1 gatcatgcta tattttaatt tgtaaagaca aaaatgacaa gcaatttatt tacagaaaac
       61 tgtacaaaag caaattaaat tatgtaaagt atttcataaa tagttggacg agtgtttaat
      121 acatttcgtc atgtttagca aagtcgcgtg catagtgaca taatcaatga tgattaattg
      181 ttctctgctt cactatcaac atccaagtag cagaacaata gtcaatgatt aacattacaa
      241 acagatcgta ccacacggaa tgcaagtgct ttaaagtgta ggaaaagttg tctgaaagta
      301 aaccctaggt agctgaacga atgatgcccc atccagatgt cacatacacc acgcctgggt
      361 caaaattaac tagctttatc atagccctga tgaacttggt tcctttcatt tcctatttca
      421 aagaggccgc tatttatcta ctactgatct aatctatagt ttgagatgtc ataggtctta
      481 agattttgca caactgtttt aagatggctg tttgattcct ggcagtttct ttctggcttg
      541 tgtatttcgt tttgtatttt cctctaaccc gatctcccag ggtagccttt caggcaaaca
      601 tcctctgtac caccttagt
//