LOCUS BG067898 779 bp mRNA linear EST 26-JAN-2001
DEFINITION H3059D12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3059D12 3', mRNA sequence.
ACCESSION BG067898
VERSION BG067898.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 779)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3059D12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3059 row: D column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..779
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3059D12-3"
/db_xref="taxon:10090"
/clone="H3059D12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 230 a 72 c 205 g 271 t
ORIGIN
1 atgtattatc atgtagatat aggcttacta ggagggtgaa tacgtaggct tgaattaatg
61 ctactgcaaa ttctagaatt gtgagtagaa gtaaaataat aaatgtaatg gtagctgttg
121 gtgggctaat atttattaat actagagtag ctcctccgat taggtgtatt aataagtgtc
181 ctgcagtaat gttagctgta accnggactg ctaatgccat tggttgaata aataggctaa
241 ttgtttcaat aataataagt attggaatta gtgaaattgg agttccttgt ggaaggaagt
301 gggcaagtga gctttttagt ttgtgtcgga agcctgtaat tacggctcca gctcatagtg
361 gaatggctat acttagattt atggatagtt gggtagtagg tgtaaatgta tgtggtaaaa
421 ggcctaggag atttgttgat ccaataaata tgattaggga aacaattatt agggttcatg
481 ttcgtccttt tggtgtgtgg attagcatta tttgtttgat aataagttta actagtcagt
541 gttggaaaga atggagacgg ttgttgatta ggcgttttga ggatgggaat aggattgaag
601 gaaatataat gatggctaca acgattggga atcctattat tgttggggta atgaatgagg
661 caaatagatt ttcgttcatt ttaattctca aggggttttt acttttatgg ttgttagtga
721 ttttggtgaa ggtgccagtg ggaatgtttg tgatgagact tttaagtgaa ataagataa
//