LOCUS       BG067888                 835 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3059D01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3059D01 3', mRNA sequence.
ACCESSION   BG067888
VERSION     BG067888.2
DBLINK      BioSample: LIBEST_008622
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced gi:12550457.
            Other_ESTs: H3059D01-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3059  row: D  column: 01
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3059D01-3"
                     /db_xref="taxon:10090"
                     /clone="H3059D01"
                     /sex="Clones arrayed from a variety of cDNA libraries"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /clone_lib="LIBEST_008622 NIA Mouse 15K cDNA Clone Set"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo  cDNA libraries (including preimplantation stage
                     embryos from  unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos,  extraembryonic part of E7.5
                     embryos, and E12.5 female mesonephros/gonad)  and one
                     newborn ovary cDNA library. Average insert size 1.5 kb.
                     All  source libraries are cloned unidirectionally with
                     Oligo(dT)-Not primers.  References include: (1)
                     Genome-wide expression profiling of mid-gestation
                     placenta and embryo using a 15,000 mouse developmental
                     cDNA microarray,  2000, Proc. Natl. Acad. Sci. U S A, 97:
                     9127-9132; (2) Large-scale cDNA  analysis reveals phased
                     gene expression patterns during preimplantation  mouse
                     develolpment, 2000, Development, 127: 1737-1749; (3)
                     Genome-wide  mapping of unselected transcripts from
                     extraembryonic tissue of 7.5-day  mouse embryos reveals
                     enrichment in the t-complex and under-representation  on
                     the X chromosome, 1998, Hum Mol Genet 7: 1967-1978."
BASE COUNT          234 a          205 c          187 g          209 t
ORIGIN      
        1 ctcttaatta ggttcatttt aataaagagt ataggagttt acaacccaaa caattatcca
       61 taaaaactat cagatacata agataaatca caccgagtga acgacagaaa cccaacagcc
      121 tgcaatgaga ggtattacag taactgctag gaatgacctt ctggtttccc ctcaagattg
      181 tttgcatgtt aaggattcct gaggcctgct gacctcagca ctccttccag gctgcttaga
      241 gctcctcact caatcaacta caaatactgc cagcagaggg cgctcatctt ccctccttga
      301 aatacgcatg gtccttggcc tctgttattt ggtttctgtt ccctaaaaat gacatcaaat
      361 gttccattca aaataatgtc ttgcaggttt tgtggtgcag gccaccactg tcctcccagg
      421 gctacccagg caccaagaag gtgcttactc gggaaggagg ctgaagcaat ggctggtcaa
      481 ttccctcacc acagacgcaa acaacttcat ttctaacttt agaggttttg gcaaagggaa
      541 cagagttgct tgtgccagct gcctgcggtc ctgtcttagc cctctgcaga tgactaagcg
      601 gaggggctgg agctaacacc gtgaaggaag caggagggta catgtctgca cacacaggcg
      661 ctgagaaact cctcacaaga atctttggta gacttgcaga acacagcagg atcaccactt
      721 ctgaaggaaa ggattttact gtctctcaaa gtgaaggtgc tggagactgg acaagggttc
      781 tcacacagtc taagcatgtg gtatcagcca acactgacct atccttgact ggtgt
//