LOCUS       BG067335                 655 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3053C04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3053C04 3', mRNA sequence.
VERSION     BG067335.2
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG067335.1.
            Other_ESTs: H3053C04-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3053  row: C  column: 04
            Seq primer: -21M13 Forward
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          182 a          160 c          150 g          163 t
        1 tggacgtcgt cgatttcctc gggaagaatg tggaagagtt ttgagttccc tttgttctcc
       61 agtgtgtctt gtacaaaata ttacatcttt aaacatatta tttacagagt tcgaacaaat
      121 agaaaatggt tacaaattac aaccaggctt ggcaagataa cagctgccct tccccccact
      181 cactggggtc aggtgtgaca aactgttcct tatttacatc tatgtttaaa aaagtctgag
      241 ttcctagaga cccggaggaa gtatataaaa actgctaaac accatcaatt catccaagcg
      301 agctatccag ggaatcgata ccaattagtc catgtctcac tccctgaata aagactctaa
      361 aattgatcct ctcagccagc cccacacccg ccctggcctg cctgctgcag tgtggggttt
      421 gggtggggag aagggagcgg atggaatggg aatagcctgg ctaagaggcc ttcctgcagc
      481 taagccagct ggccggtgta gcgccactct aaggctttga aagaacacac aggtcctcct
      541 gggaggacct cctcctccac aggactacag aatggggaaa gaagactcag tgagaacagt
      601 gtttgttact ctgctcttaa aactctgtcc tgggtgcaaa cccactctca acagt