LOCUS BG067012 747 bp mRNA linear EST 12-MAY-2010 DEFINITION H3049E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3049E12 3', mRNA sequence. ACCESSION BG067012 VERSION BG067012.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 747) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067012.1. Other_ESTs: H3049E12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3049 row: E column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..747 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3049E12-3" /db_xref="taxon:10090" /clone="H3049E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 264 a 151 c 117 g 215 t ORIGIN 1 agtagacaag aattacatat ttgttttatt gacaccaggt ataaacttgt gttaaacaag 61 taaacccagt gaatagcacc atggttaaga ctctagcctg ctttaccatt tacaattttg 121 taagtcaaca ggggatcaaa tgtcttacat ctatctacct gtgatcttta aatacaaaaa 181 aaaaagattg gataattaac cctgcatagt tataaatcta taaccagtcc acacacacaa 241 caggctctgc atatacacta gaacggatga caaagtctta aaaagctgta agaaatacat 301 ttctaactag agaagctgca aatttcaaaa gggtatggaa aaaaactaaa tccaagactt 361 ccctcttatg ttcaaagaaa tagactttct gtaaaaatat atacaatttt tacagacaaa 421 tacatttcat aaattttctt aaaaatcgag gctgttcagt tttgtaacta attagtgaac 481 cttaagtcaa gtactttttg gccatcacta ggctaataaa cttaagaatc atccaaacca 541 agccaaagta gacagggcta aaagtcctga gcacttggga ctttgtcaag cacaccctgg 601 actgctcaga ggcctccctt cacattcctg tacagcactg cagcttctag tcatcgtttg 661 gtgacttgta ggttacaaat ccattttaca caagtcacca acgttgttaa ttagggcttc 721 tctctcaaca ctctgagcag agtaatg //