LOCUS BG066552 778 bp mRNA linear EST 12-MAY-2010
DEFINITION H3045C07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3045C07 3', mRNA sequence.
ACCESSION BG066552
VERSION BG066552.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 778)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066552.1.
Other_ESTs: H3045C07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3045 row: C column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..778
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3045C07-3"
/db_xref="taxon:10090"
/clone="H3045C07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 253 a 149 c 140 g 236 t
ORIGIN
1 cattttgttt tatttgtttt tggttcagca taacttggaa catttgaaag cttttcaacc
61 taaatgtggg gggaaacagg taaggcattt atttttgcac aaaactagca ttcctaatag
121 tgcaaatgaa tctggtgcct cctaaaatgg tgagaggcca tatactcact agattaattt
181 agggtttctt tctatggctt cacagattat tcctctataa attctattct cacccagagc
241 tgttgctgta atcaaaagat aactagatca gagtccagcc ttttcctgta tccagcaaaa
301 ggtttttgct cagatgcaaa acatctcatt tttaaattag gctacaagca aaagtacaac
361 ttggactcct gagaatcttc gaataccagt agcaacgagc tgatctggac agcagtctca
421 aaggagctcc atagctcaaa aaatagtcaa tctaaaacct gggtcatcct ttcccctccc
481 ctgaattagc tactacaatg tacttgagaa gttaagactt agtttgaaaa tgtcatacat
541 acgatgagat gcatcagatc tgtcatttga tcatctgata aaacttgcat agcaagatgg
601 aattcaataa gccttttctg ttgttgacaa tcatctggaa gacttgaaag cttctacaaa
661 ctcaaagcat tgctcactaa atatcagaat gtccctgcca catggaaaca gatgtataaa
721 tgagtcagga atctgaacat tgatattaat aaactaaggg taatttaggg gaaactgg
//