LOCUS       BG065462                 542 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3031D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3031D08 3', mRNA sequence.
VERSION     BG065462.2
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG065462.1.
            Other_ESTs: H3031D08-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3031  row: D  column: 08
            Seq primer: -21M13 Forward
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          145 a          132 c          110 g          155 t
        1 aaaaaagatt tatttattta ttatatgtaa gtacactgta gctgtcttca gacactccag
       61 aagagggcac cagatcttgt taaggatggt tgtgagccac catgtggttg ctgggaattg
      121 aactcagtac ctttggaaga gcagtcagag ctcttacctg ctgagccatc ttgccagccc
      181 agttcttttt ctttttaagc atctttaatc ccagcttcac tttctctgtc aatcatcctt
      241 aggccttata cagatcttag tggaatccac tctatcaacc acatggagag agaaagctct
      301 aagagcagat gtaattggct atgttcagag cagtctgctc atggcagctg taggacacag
      361 agaatggcta tattttcctc ctttgtacag ccctccatgg ctcaagacac cttacttgga
      421 tatcctgccc cgtcaactga agcccagtta agcagctctt catttctgca caagacacag
      481 tctggctgag aataaaacaa ctgtcagaat agtggctagg gccctgcacc tgttcccacc
      541 aa