LOCUS BG065273 669 bp mRNA linear EST 12-MAY-2010
DEFINITION H3029B12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3029B12 3', mRNA sequence.
ACCESSION BG065273
VERSION BG065273.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 669)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG065273.1.
Other_ESTs: H3029B12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3029 row: B column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..669
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3029B12-3"
/db_xref="taxon:10090"
/clone="H3029B12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 214 a 174 c 146 g 135 t
ORIGIN
1 ctttctcatg tgtgtttatt gagcgccgac agagcccgtg gtactgtgct caggattcag
61 agccacagtc tagtcagaac gagaccagag ctagtccaca gaatgtttca tagctgtgta
121 acatctgtta cgggatcagg atggggaaaa ctaatcacga aacaaattcc tattcaaaag
181 caacagaacc tcctctgaga gtaatataga ttctaggcac acatactcac cacacacaca
241 cacacacaca cacacacaca cacacaccct cagaatgaac acccaactat gcccacttag
301 ttgtatatgc atgcatatac agatgttggt gggcaagtaa attaatatat atgctgtaca
361 cacacacact ggcacacgca cacacataca ctctcacaca aaccaccaca cagacagaaa
421 atctggctta tgctatcaaa gacactatgt ctccaggttc tagcggaagt aacctgtcct
481 agatgtctgc agcatgcaaa acttgaaaga tccagagagt cagtttagga gaacaatggg
541 ggcacatggg tatgcttcca tcctcagagc cccagccata gcacggaagg caacaacaaa
601 gcctttgtga agggctccag gggcagagca gaggacctgg aggctccctg tgatggcggg
661 ggccaggag
//