LOCUS BG065254 725 bp mRNA linear EST 12-MAY-2010 DEFINITION H3029A03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3029A03 3', mRNA sequence. ACCESSION BG065254 VERSION BG065254.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 725) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG065254.1. Other_ESTs: H3029A03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3029 row: A column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..725 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3029A03-3" /db_xref="taxon:10090" /clone="H3029A03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 189 a 183 c 173 g 180 t ORIGIN 1 gcacaggaag agacatttat tttgggagca gtctttgcaa agataaagct attaacacta 61 aagtgatccc ccaccccccc cacacagaaa tcataagctc ttgtgaagta ggaaacattc 121 tcaaggcgct aaggcacgca ggaacaagat gggtggcagt ttccttggct tagttagagc 181 ttggctttct tcttaaaaga ttcctttatc ctcttgacaa tagaaaggtc cccagctcct 241 acaggccagt tgtcatgatc cggtatgctg aaggcgtgag tgtacagagc ctggcctcca 301 tccacattga tcaactgtcc agtgatatag gaagccgcag gggacagcag gaaacatacc 361 agaggggaga tctcctcggg gactccaagg cgcttagctg ggatactgtc aaaggccatc 421 tcaaataagg tttgtcccat ttcgccatag ttgtcaacag cagtctggga gtaaatggtc 481 ccaggagcga cacagttgat cctcactccg ctgctggccc acgccaaagc catggatttt 541 gtgaggttgt aaacaccttc tcttgccgct ccagtgtgcg cggctgttgg aaacccatta 601 ttaagaagga caatgatatt gacaatcgac cctccgtgct ctctcatcca ggaattgtaa 661 acttctttgc acatgtagaa ggtgcctgtc aggttggttt ctatcacagc atgccatccc 721 ttcgc //