LOCUS BG064537 782 bp mRNA linear EST 12-MAY-2010 DEFINITION H3020H06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3020H06 3', mRNA sequence. ACCESSION BG064537 VERSION BG064537.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 782) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG064537.1. Other_ESTs: H3020H06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3020 row: H column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..782 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3020H06-3" /db_xref="taxon:10090" /clone="H3020H06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 225 c 216 g 180 t ORIGIN 1 cttttttggt aataaaaatt ttcttggttt aggcgaaata ctctgtgcaa cgcagtaccg 61 agggggagcc ctgccccccc ccagccccct ccccttcctt tgcctcctgc aggggggaag 121 ggagggactt ctgggcaggg gtgaatgtgg gagggagaag aaccacttcc catcccccac 181 aaacatggaa gaaaaaaact gagaagggcc tggaagggga caagtgccag caggggagac 241 acaccaattc atcctgcagt ttctcccagg gggatgcagg gttggcccaa gcagccaagt 301 ccactggtgg gcctctgctg cccctcctga cagcctgtta aagcttcagc tcattggcta 361 ttttggaggc aatgtttttg aaggccatgg aagtgcccga tatccgctta aaccgaacac 421 cgttgagaga cagccggggc agtttgcaca cctccatctc ccactgcaca aagttctcgt 481 ggcctggtgt gccatgcagc acagtagcat gtaccgctcg tgcagctcgc tttggcagct 541 gttggcgtcc agcaccttgc ggatctcccg catcatctca ttgggctcca tagagctcgt 601 ggtcttcatg ctccaggtga agcgcaggga gcgaggcttg gctcccgaaa ctcctccttg 661 tccttgtcag tgcctccact gcctaccacg tgaggtctga gcgtctccac tcggtctttg 721 ctttcaggtt cattcaggtt cctgcggaca aacttggagg tgaacttgct gaagatgctg 781 cc //