LOCUS       BE686999                 349 bp    mRNA    linear   EST 07-JAN-2011
DEFINITION  uw08g12.x1 Soares mouse 3NbMS Mus musculus cDNA clone IMAGE:3416134
            3', mRNA sequence.
ACCESSION   BE686999
VERSION     BE686999.1
DBLINK      BioSample: SAMN00155158
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 349)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:1091946.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:3416134"
                     /sex="male"
                     /tissue_type="Spleen"
                     /clone_lib="SAMN00155158 Soares mouse 3NbMS"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTGTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through
                     three rounds of normalization, and was constructed by
                     Bento Soares and M.Fatima Bonaldo."
BASE COUNT          125 a           64 c           59 g          101 t
ORIGIN      
        1 ttttttttta agggaaaaac ttcaaattaa taattttaat aaaagcatat tatacacatg
       61 catgcaacaa tatcttttta tcaacataga attataaaat atgtctagta ctcacaaagc
      121 tacccatgtg gctcagctgc ctcatgtaaa ttgtcagaaa tggttcttta gaaggaaaac
      181 gagaaagact agaacagaaa gttctgtctc cgtattaaaa taaaaacgtg aacatactga
      241 gaagcagggg gagattatag cgtaactctc cctaaactct gtgtggctgc tcaagtcgtc
      301 acatcttcag tgtgagcaca caatggtaaa agtcactcac ctttgccta
//