LOCUS BE686999 349 bp mRNA linear EST 07-JAN-2011 DEFINITION uw08g12.x1 Soares mouse 3NbMS Mus musculus cDNA clone IMAGE:3416134 3', mRNA sequence. ACCESSION BE686999 VERSION BE686999.1 DBLINK BioSample: SAMN00155158 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 349) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:1091946. FEATURES Location/Qualifiers source 1..349 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:3416134" /sex="male" /tissue_type="Spleen" /clone_lib="SAMN00155158 Soares mouse 3NbMS" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through three rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 125 a 64 c 59 g 101 t ORIGIN 1 ttttttttta agggaaaaac ttcaaattaa taattttaat aaaagcatat tatacacatg 61 catgcaacaa tatcttttta tcaacataga attataaaat atgtctagta ctcacaaagc 121 tacccatgtg gctcagctgc ctcatgtaaa ttgtcagaaa tggttcttta gaaggaaaac 181 gagaaagact agaacagaaa gttctgtctc cgtattaaaa taaaaacgtg aacatactga 241 gaagcagggg gagattatag cgtaactctc cctaaactct gtgtggctgc tcaagtcgtc 301 acatcttcag tgtgagcaca caatggtaaa agtcactcac ctttgccta //