LOCUS       BE518338                 434 bp    mRNA    linear   EST 08-AUG-2000
DEFINITION  WHE0809_F07_K13ZS Wheat vernalized crown cDNA library Triticum
            aestivum cDNA clone WHE0809_F07_K13, mRNA sequence.
ACCESSION   BE518338
VERSION     BE518338.1
DBLINK      BioSample: SAMN00160091
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D.,
            Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J.,
            Seaton,C.L. and Tong,J.C.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - Vernalized crown cDNA library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Stratagene SK primer.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Chinese Spring"
                     /db_xref="taxon:4565"
                     /clone="WHE0809_F07_K13"
                     /tissue_type="Crown tissue of seedling"
                     /clone_lib="SAMN00160091 Wheat vernalized crown cDNA
                     library"
                     /dev_stage="Five-week old seedling"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and
                     grown at 4 C for 5 weeks. The tissue, total RNA, and
                     poly(A) RNA were prepared, a cDNA library was made, and
                     the cDNA clones were in vivo excised to give pBluescript
                     phagemids in the TJ Close lab (Choi, Close, Fenton) at the
                     University of California, Riverside. Plasmid DNA
                     preparations and DNA sequencing were performed in the OD
                     Anderson lab (all other authors)."
BASE COUNT           90 a          117 c          125 g          102 t
ORIGIN      
        1 agcgttgtgt gcgtgaccgg cgcagggggt ttcgtcgcct cgtggctcgt caagctcctc
       61 ctctccaagc cccactacac cgtccgcggc accgtgcgcg atcccggtaa tgccaagaat
      121 tctcatctca aggcgctaga aggtgctggg gaaaggctgc agctgcttag cgctgacctg
      181 atggactacg acagcattgc gtcggcggtt gccggctgtg agggcgtctt ccatgttgct
      241 agccctgtcc cttctggcag atcaacgaat cccgaggaag aagttatagc ccctgctgta
      301 acaggcacac tgaatgtgtt gaaggcttgc tacgaggcaa aagttaagcg agttgtcata
      361 gtgtcttcat gtgccgctgt gttcaataat cctaactggc ctaagggcaa agtatttact
      421 gaagacagct ggtc
//