LOCUS BE518338 434 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0809_F07_K13ZS Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0809_F07_K13, mRNA sequence. ACCESSION BE518338 VERSION BE518338.1 DBLINK BioSample: SAMN00160091 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 434) AUTHORS Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D., Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L. and Tong,J.C. TITLE The structure and function of the expressed portion of the wheat genomes - Vernalized crown cDNA library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Stratagene SK primer. FEATURES Location/Qualifiers source 1..434 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Chinese Spring" /db_xref="taxon:4565" /clone="WHE0809_F07_K13" /tissue_type="Crown tissue of seedling" /clone_lib="SAMN00160091 Wheat vernalized crown cDNA library" /dev_stage="Five-week old seedling" /lab_host="E. coli SOLR" /note="Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 90 a 117 c 125 g 102 t ORIGIN 1 agcgttgtgt gcgtgaccgg cgcagggggt ttcgtcgcct cgtggctcgt caagctcctc 61 ctctccaagc cccactacac cgtccgcggc accgtgcgcg atcccggtaa tgccaagaat 121 tctcatctca aggcgctaga aggtgctggg gaaaggctgc agctgcttag cgctgacctg 181 atggactacg acagcattgc gtcggcggtt gccggctgtg agggcgtctt ccatgttgct 241 agccctgtcc cttctggcag atcaacgaat cccgaggaag aagttatagc ccctgctgta 301 acaggcacac tgaatgtgtt gaaggcttgc tacgaggcaa aagttaagcg agttgtcata 361 gtgtcttcat gtgccgctgt gttcaataat cctaactggc ctaagggcaa agtatttact 421 gaagacagct ggtc //