LOCUS BE517606 570 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0628_B06_D12ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0628_B06_D12, mRNA sequence. ACCESSION BE517606 VERSION BE517606.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 570) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..570 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0628_B06_D12" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 113 a 199 c 146 g 112 t ORIGIN 1 gcggccgcgt cgacactaat tcacttcctt cggatcccgc tcgtgccacc aagctctgct 61 cccgctaaga tgtcgacggt gacccgtgcc aacctcgacc agaggctcgc cgccgccaac 121 cgctgctcca gaggtaacca ccgacgaccg gttccggcca cagcccagca ctgctctgct 181 cctatatccc aaccatccat tgcagcttat gtgtctgctg aaacttgatc ttgattaaca 241 ttcctgcgtt ggtctgaaac tgcagaggct gccatggcgg gagccaaggc cgcggccgtc 301 gccaccgtcg ctgccgcagt acccacagta agtagccagc tagccattca gttccagagt 361 tcatagaaca catctcatga atcaacgcgt tacctgtact gtaatttcag ctggcgagcg 421 tgaggatgct gccgtgggcg agggcgcacc tcaaccccag cgggcaggcg ctcatcatct 481 ccacggtcgc cgggatggcc tacttcatcg tcgccgacaa gaccatcctc tccatggcca 541 ggaggcactc cttcgacgtc gacgcggccg //