LOCUS BE517432 569 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0626_D10_G20ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0626_D10_G20, mRNA sequence. ACCESSION BE517432 VERSION BE517432.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 569) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..569 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0626_D10_G20" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 102 a 162 c 186 g 119 t ORIGIN 1 ggccgcgtcg accaccgtct agctagctct tgttaggtcg atcggcaatg gcgtccacca 61 aggctctctt cgtgctcgcc gttctcctcg cgtcggccgt cctcctcgcc gccgcggcct 121 ccgagcaaac tcatgacaag gaggggaagg tggccaccaa cggtgccggc gtccaggacg 181 gctggggtgg cggcggccgc ggcggagggt acccgggccg cgggggcggc ggctacgggc 241 cctgcggccg gtggggctgc tgccgccgtg ggtaccacgg cgactgcatc cgatgctgca 301 gggccgccaa cgacgtcccg gaggccatgg accgcactga ggtgcacaac tagctagctg 361 gcagctggct atggcttggc tagcaagcag gacccatgat caatctggaa gctagcttct 421 tccagggtac tgagtaaaat aaatctccgg tttagttcat gtgatctatg tatgtgcgcg 481 gatgaacgta tgtatctacg tacatacgtt cgtagtcctc ttcctttgtt tggttggaac 541 aataataaga aaatgtgatg cgtgttgag //