LOCUS BE517269 526 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0616_E10_J20ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0616_E10_J20, mRNA sequence. ACCESSION BE517269 VERSION BE517269.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 526) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..526 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0616_E10_J20" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 114 a 117 c 120 g 175 t ORIGIN 1 cgcggccgcg tcgacttttt tttttttttt ttgaagctca tgcatttcaa tgatttgatt 61 gaatctaatt ttgaaaaaat gtttggtgca gatattgttt gctcccctaa agatacaagt 121 gctcagctgg ggcaactaca aacaagactc ttccctgtct cacaaagact tgttgaatgt 181 ccacaggaac tacgatttcc gcgcaatcat ggcgaactcc acggtcacaa gcatgatgtc 241 atggcagtgg cctctaaaat ttgggtctgg tgttctgctt ttgaatgggc ctgttcggac 301 caactggttt ggtgatggtt atcgttgaat taggtcgatg aattgtcgtc gtcctcgatg 361 tctgttcgct aggaaaggtc agccttgctg gctgtgatga ccttgcaaca gtgttccttt 421 ggatagcagc ttgcattttc gcccgactgt caacctccat ccttgccttt ttccgccttt 481 ctcattcaag agcagctcgg cgttgtcaga aataattgta ttgatt //