LOCUS BE517054 547 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE623_D03_H05ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE623_D03_H05, mRNA sequence. ACCESSION BE517054 VERSION BE517054.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 547) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..547 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE623_D03_H05" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 133 a 169 c 141 g 104 t ORIGIN 1 cgcggccgcg tcgaccagat atttgtgaag accctcaccg gcaagaccat caccctggag 61 gtggagtcat ctgacaccat cgacaacgtc aaggcgaaga tccaggacaa ggagggcatt 121 cccccggacc agcagcgtct tatctttgct ggcaaacagc tcgaggatgg ccgcaccctt 181 gcggactaca acatccagaa agagtccacc ctccacttgg tgctcaggct ccgtggtggt 241 atgcagattt ttgtcaagac cctgaccggc aagaccatca ccctggaggt cgagtcgtct 301 gacaccattg acaatgtgaa ggccaagatc caggacaagg agggcattcc cccggaccag 361 cagcgtctca tcttcgctgg caagcagctt gaggatggcc gcacccttgc ggattacaac 421 atccagaagg agtccaccct ccaccttgtc ctccgccttc gtggaggcat gcaaatcttt 481 gtcaagaccc tcactggcaa gactatcacc ttggaggtgg agtcatctga caccatcgac 541 aacgtga //