LOCUS BE516864 478 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE0622_B06_C12ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE0622_B06_C12, mRNA sequence. ACCESSION BE516864 VERSION BE516864.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 478) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..478 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE0622_B06_C12" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 111 a 128 c 126 g 113 t ORIGIN 1 gcggccgcgt cgactttttt tttttttttt tttggtaaac atataatgaa gtcttcatat 61 caaaatccaa ggcacagacc cataaagatg cacattaaag ggttgaaaac gctcatccac 121 gacatcatac gaataataaa gcagtcttca gatagcatat cctaacagaa ctggggatgg 181 taataaaact aggatatcaa gagcagtatg gcaaccggca gatcccaagc ttaatcgatc 241 tcctggagcg aggcaccggt ggccgtggcg cccccagcgg cggcggcgac gtcctccgcc 301 gtgaactttc ccttggcctt gtcagcagcg cggccgcgag ccttgcggtc gaggagggcc 361 ttgcggtcct tgtcgagctt gagcttggtg acgatcacct tgctggggtg gatgccgacg 421 ttcaccgtgg atccattgac cttctccctg gtgatccgct ccacgtggat gacccagc //