LOCUS BE516702 547 bp mRNA linear EST 08-AUG-2000 DEFINITION WHE619_H11_P21ZA Wheat ABA-treated embryo cDNA library Triticum aestivum cDNA clone WHE619_H11_P21, mRNA sequence. ACCESSION BE516702 VERSION BE516702.1 DBLINK BioSample: SAMN00160090 KEYWORDS EST. SOURCE Triticum aestivum (bread wheat) ORGANISM Triticum aestivum Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum. REFERENCE 1 (bases 1 to 547) AUTHORS Anderson,O.D., Chao,S., Han,P.S., Hsia,C.C., Johnson,R.R., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J., Seaton,C.L., Tong,J.C., Verhey,S.D. and Walker-Simmons,M.K. TITLE The structure and function of the expressed portion of the wheat genomes - ABA-treated embryo library JOURNAL Unpublished COMMENT Contact: Olin Anderson US Department of Agriculture, Agriculture Research Service, Pacific West Area, Western Regional Research Center 800 Buchanan Street, Albany, CA 94710, USA Tel: 5105595773 Fax: 5105595818 Email: oandersn@pw.usda.gov Sequence have been trimmed to remove vector sequence and low quality sequence with phred score less than 20 Seq primer: Clontech Matchmaker 3' AD primer. FEATURES Location/Qualifiers source 1..547 /organism="Triticum aestivum" /mol_type="mRNA" /cultivar="Brevor (soft, white, winter, common wheat)" /db_xref="taxon:4565" /clone="WHE619_H11_P21" /tissue_type="Seed embryo" /clone_lib="SAMN00160090 Wheat ABA-treated embryo cDNA library" /dev_stage="Mature dormant seeds" /lab_host="E. coli DH12S" /note="Vector: pGAD10; Site_1: EcoRI; Site_2: XhoI; Embryos were cut from mature, dormant seeds and imbibed in 25 microM ABA (abscisic acid) in 5 mM Mes buffer, pH 5.7, for 12 hr at 22 C. The tissue, total RNA, and poly(A) RNA were prepared by Steven Verhey in M.K. Walker-Simmons's lab (USDA-ARS, Washington State Univ., Pullman, Washington 99164-6420. A cDNA library was made by Clontech using a combination of random and oligo dT primers. Library was plated and archived by Russell Johnson (Colby College, ME/Walker-Simmons' lab). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors)." BASE COUNT 138 a 141 c 144 g 124 t ORIGIN 1 cgcggccgcg tcgacttttt ttttttgaaa actagcatct gattagatga tcatagaaca 61 aagggaacag agagagcaga aagatagtca tggataaagc cacggtggca gcaagagaac 121 acataataga cattcattgc taactacact acatactgga aacttgcgaa taagccggca 181 ctagtctagc aagcggcagc tgcacgcaat ggcggagtat ctagcttgat cctctccctt 241 gtgcccctcc agcgccttcc atggagtaga agctccaagg tctctggttg atcactgctt 301 ggctttccgg ctaccattgc gagacctctt tcggctctcc ttggagccgc caggcttcgg 361 ttgggtagac cgggcgttag gaataggcac acgatgctca ctagccaaag cagggaaacc 421 agcggcaggg tttccgaaag tacggcagac tcctgcatgg ctgtgtgagc accaataggc 481 ctatccttca acatgttctg gcgcagtgcc atgttctcag ctgcgcagca ctgaagtgcg 541 tagctga //